Studies on Biological Activity of Wood Extractives (X VIII) -Isolation and Antioxidant Activity of Chemical Constituents from Maackia amurensis-

The dried barks of Maackia amurensis were ground, extracted with 95% EtOH, concentrated, and fractionated with a series of light petroleum ether, dichloromethane, ethyl acetate and water on a separatory funnel. Each fraction was concentrated, then a portion of dichloromethane and ethyl acetate soluble was chromatographed on a Sephadex LH-20 and silica gel 60 column using a various solvent system as eluents. The isolated compounds were identified by cellulose TLC, , , COSY, NOESY, HMQC, HMBC, FAB and EI-MS. The structures were determined as: 7-O--D-glucopyranosyl-4'-methoxyisoflavone, 7-O--glucopyranosyl(1'''->6'')--D-glucopyranosyl-4'-methoxyisoflavone, 7-O--D-glucopyranosyl(1''''->6''')--D-glucopyranosyl(1'''->6'')--D-glucopyransoyl-4'-methoxyisoflavone, 7-O--D-glucopyranosyl-4', 6-dimethoxyisoflavone. The Free radical scavenging activity using DPPH of the isolated compounds were similar with that of BHT but lower than of -tocopherol.