Rapid and prognostically valid quantification of immunohistochemical reactions by immunohistometry of the most positive tumour focus. A prospective follow‐up study on breast cancer using antibodies against MIB‐1, PCNA, ER, and PR

The prognostic significance of immunohistochemical markers for cell proliferation [MIB-1, proliferating cell nuclear antigen (PCNA)) and hormone receptor analysis (oestrogen receptor (ER), progesterone receptor (PR) was tested by means of immunohistometry in a series of 103 breast cancer patients in comparison with the lymph node status N, the tumour size T, histomorphological grading and the biochemical ER and PR status. Immunohistochemical reactions were performed on 2 μm sections from paraffin-embedded tissue, using an indirect peroxidase method. The proportion of immunostained tumour cell nuclei was determined using a TV-image analysis system. Measurements were performed using a 20 x objective on 40 viewing fields (1.94 mm 2 , MIB-1 and PCNA) or 20 viewing fields (0.97 mm 2 , ER and PR). The mean immunopositivity of all viewing fields and the value of the most immunopositive viewing field (MIB-1 max , PCNA max , PR max , ER max ) were calculated. The mean values and the maximal values were highly correlated (r=0.903, P 5 revealed significantly different 7.5-year survival probabilities (P<0.0001). The simplicity of the PI makes it a clinically useful, routinely applicable, and understandable parameter in the surgical pathology of breast carcinoma.