Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations

Chromosome regions determining kernel high oil content were identified by RFLP analysis of individual plants from C0 and C9 selection cycles of two maize synthetic populations -DS7u and YuSSSu. Identification of chromosome regions was done with informative RFLP markers (that were identified with BSA earlier) using a single plant approach - analysis of individual plants. This analysis revealed the number of plants carrying alleles that endured frequency alterations during selection process. Statistical analysis (χ2 test) revealed chromosome regions that comprise putative QTLs affecting expression of kernel high oil content of analyzed maize populations. Four regions on chromosomes 1, 6, 7 and 8 were identified in both DS7u and YuSSSu populations. Additional four regions on chromosome 4, 9 and 10 were detected only in DS7u population.