Development of drug screening model based on reporter gene and the signal transduction of PPAR#gamma#

The expression levels of reporter gene induced by rosiglitazone in 293T cells was the highest, up to 4.9 fold, and in dose dependent manner. The Z factor was 0.72. The expression levels of luciferase in 293T cells induced by the ligands of nuclear receptors were about 1.0 fold. No effect of rosiglitazone on the proliferation of 293T was observed. The expression of luciferase in 293T cells co-transfected with the recombinant vector PPERE-tk-luc~(+) and pCMX-PPAR#gamma# vector could be induced significantly. The preferable speciality and stahbility of the cellular model make it well suited for establishing high-throughput screening systems for agonists of PPAR#gamma#.