Absorption mechanism of oxymatrine in cultured Madin–Darby canine kidney cell monolayers

AbstractContext Oxymatrine (OMT) is beneficial to human health by exerting various biological effects. Objective To investigate the absorption mechanism of OMT and discover absorption enhancers using Madin–Darby canine kidney (MDCK) cell monolayers.Materials and methods Concentration effects on the transport of OMT were measured in the range of 1.0 × 10−5–1.0 × 10−3 M in 2 h. Then, the effect of time, direction, temperature and pH on the transport of OMT at 10−4 M was studied. Moreover, Papp of OMT was determined in the absence/presence of cyclosporine and surfactants at 100 μM to further confirm the relative transport mechanism.Results The Papp AP→BL ranged from (3.040 ± 0.23) × 10−6 to (3.697 ± 0.19) × 10−6 cm/s as the concentration varied from 10−5 to 10−3 M. OMT showed similar Papp at 4 and 37 °C (p > 0.05). Increasing the apical pH 7.4 and 8.0 resulted in Papp versus pH 5.0 (p < 0.01). Furthermore, in the presence of cyclosporine and surfactants including sodium citrate, sodium dodecyl sulphate (SDS)...