Simultaneous Analysis of Ibotenic Acid and Muscimol in Toxic Mushroom, Amanita muscaria, and Analytical Survey on Edible Mushrooms

A convenient analytical method for ibotenic acid (IBO) and muscimol (MUS) in a toxic mushroom, Amanita muscaria (A. muscaria), was developed. IBO and MUS in the mushroom were extracted with 70% methanol. After filtration, IBO and MUS in the extract were determined by high performance liquid chromatography (HPLC) with a UV detector set at 210nm. The HPLC system adopted was ion-pair chromatography in the reverse-phase mode on an IRICA RP-18 (C18) column (4.0mm×25cm) with sodium dodecyl sulfate as a counter ion. Recoveries of IBO and MUS added to the sample were more than 98%, and the minimum detectable concentration of IBO or MUS was about 1ppm. The concentrations of IBO and MUS in A. muscaria ranged from 258 to 471ppm and from 18 to 27ppm, respectively. Neither of the compounds was detected in commercial edible mushrooms.