Optimized drowning procedures for pulmonate land snails allowing subsequent DNA analysis and anatomical dissections

We present a method for drowning landsnails from temperate climate that is suitable for large-scale studies that include anatomical, morphological and DNA-based methods. The snails were drowned for 30 min at room temperature and subsequently incubated in 37 °C water for 30 min. The water was then drained and the snails stored in 80% ethanol.