Analysis method for measuring pyrroloquinoline quinine content through ion pair chromatography

The invention discloses an analysis method for measuring pyrroloquinoline quinine (PQQ) content through ion pair chromatography, and belongs to the technical field of chromatographic analysis. The PQQ produced through synthesis or fermentation is quantitatively analyzed by ion pair high performance liquid chromatography, the obtained regression equation is y=4E+06X+627846, R =0.9995, and is linear in a test range of 2 to 12 mu g/mL, and the minimum detectable amount is 2.0ng (S/N is more than or equal to 3). The method is simple, and possibly generated impurities in the preparation process are effectively separated.