Polymer−Peptide Conjugates Convert Amyloidinto Protein Nanobundles through Fragmentation and Lateral Association

The assembly of proteins into amyloid fibrils has become linked not only with the progression of myriad human diseases, but also with important biological functions. Understanding and controlling the formation, structure, and stability of amyloid fibrils are therefore major scientific goals. Here we utilize electron microscopy-based approaches combined with quantitative statistical analysis to show how a recently developed class of amyloid modulators—multivalent polymer–peptide conjugates (mPPCs)—can be applied to control the structure and stability of amyloid fibrils. In doing so, we demonstrate that mPPCs are able to convert 40-residue amyloid β-fibrils into ordered nanostructures through a combination of fragmentation and bundling. Fragmentation is shown to be consistent with a model where the rate constant of fragmentation is independent of the fibril length, suggesting a local and specific interaction between fibrils and mPPCs. Subsequent bundling, which was previously not observed, leads to the form...