Expression of Protein in Bauxite Exposed Mine W orkers by Induction of Heme Oxygenase-1

The primary aluminium industry consists of mining of bauxite. Employees in bauxite mines are exposed to a number of potential respiratory irritants. This study aims to investigate the early diagnostic biomarkers for the identification of an adverse health effect of bauxite exposure. Occupationally bauxite dust exposed mine workers and control subjects were selected for comparison. Significant changes were observed in Bilirubin, Serum glutamic pyruvic transaminase, Cholesterol and Triglycerides. Sodium Dodecyle Sulphate Poly Acrylamide Gel Electrophoresis was performed on bauxite exposed workers showing Heme Oxygenase -1 induction in and above normal range. The results of the repeated performance of this experiment showed differential expression of approximately 18.06 kDa proteins in 80 % bauxite dust exposed workers which were having high range of Heme Oxygenase -1. This study can help to assess correlation of protein expression in HO-1 induced subjects in association with biochemical parameters for identification of biomarkers. The present study aims to determine serum HO-1 levels in bauxite dust exposed workers and relative protein expression by HO-1 induction.