Abstract 728: Enhancement of survival and migration of neutrophils by tumor associated a2 isoform vacuolar ATPase

Tumors associated neutrophils (TAN) regulate the tumor environment by directly promoting tumor progression and represent a potential therapeutic target for cancer treatment. Identifying tumor associated factors that promote neutrophil recruitment and survival will uncover new targets for cancer therapy. In tumor cells, a secreted peptide from the N­terminal domain of a2­ isoform vacuolar ATPase (a2NTD) promotes the protumorigenic properties of neutrophils which in turn, enhance tumor progression. In order to investigate the regulatory role of a2NTD on neutrophils survival, we stimulated freshly isolated neutrophils from healthy volunteers with recombinant a2NTD. a2NTD treatment delayed apoptosis in neutrophils and led to 2.1 fold increase in the percentage survival (P Citation Format: Safaa A. Ibrahim, Arpita Kulshrestha, Mukesh K. Jaiswal, Gajendra K. Katara, Magdy A. Amin, Kenneth D. Beaman. Enhancement of survival and migration of neutrophils by tumor associated a2 isoform vacuolar ATPase. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 728.