Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods

BACKGROUND: Antioxidants are substances that can inhibit the oxidation process, so that they have a protective effect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto-Botto leaves (Chromolaena odorata L.). AIM: This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods. METHODS: The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods. RESULTS: The n-butanol fraction of Botto-Botto leaves had the highest antioxidant activity, namely the strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 μg / mL and 60.885 μg / mL, respectively. CONCLUSION: The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction.