ROLE OF ANTIOXIDANT ON FRUCTOSE-INDUCED HYPERGLYCAEMIA

The present study tries to investigate oxidative stress - hyperglycaemia relationship through studying role of ROS and oxidative stress in the pathogenesis of type 2 diabetes mellitus and role of NAC as antioxidant in normalizing that disorders. 80 rats were randomly assigned into four equal groups, 20 rats each; Control group (C) fed on the balanced ration and ordinary drinking water ad-libitum, Fructose fed group (F) fed on the balanced ration and fructose 10% (100g/L) in drinking water, Fructose and N-acetylcysteine group (FN) fed on the balanced ration and fructose 10% (100g/L) in drinking water for 4 weeks then injected I/P with N-acetylcysteine in dose of 200 mg/Kg b.wt. daily for the last six weeks and N-acetylcysteine and Fructose group (NF) fed on the balanced ration and ordinary drinking water and injected I/P with N-acetylcysteine for 4 weeks then given fructose in drinking water for the last six weeks. Blood samples and liver specimens were collected from ten animals from each group after 2 and 10 weeks. The obtained results revealed that fructose administration increased hepatic MDA content and GSPx activity and plasma insulin, insulin resistance and glucose levels. Conversely, excess fructose decreased hepatic GSH content, expression of insulin-receptor's protein gene, SOD, hexokinase and G6PD activities. Those disorders originated a case of type 2 diabetes. Injection of NAC either as treatment or protective relieved all that disorders towards normal condition through its antioxidant influ- ence.