Improved bioavailability of vitamin D3 using a β-lactoglobulin-based coagulum

Abstract Vitamin D 3 (D 3 ) was encapsulated within a water-soluble matrix, formed by promoting the βlg/D 3 complex by acidification. The capacity of the βlg-based coagulum to increase the long term stability of D 3 in cold storage, upon exposure to intensive UV-light, and in the presence and absence of intestinal proteases, was evaluated. Additionally, the impact of the sequestration of D 3 within the matrix of βlg-based coagulum on its bioavailability was determined in vivo with force-fed rats. The water solubility, long-term storage and UV-light stability of D 3 were significantly increased ( p 3 , increased uptake of D 3 and subsequent enhancement of the bioavailability of D 3 in rats.