Kit for detecting I680T site mutation of RB1 gene

The invention relates to a kit for detecting I680T site mutation of an RB1 gene. The kit comprises a specific primer for detecting I680T site mutation of the RB1 gene, the upstream primer nucleotide sequence of the specific primer is as shown in SEQ ID No.1, and the downstream primer nucleotide sequence of the specific primer is as shown in SEQ ID No.2. The relationship between I680T site mutation of the RB1 gene and prognosis of a non-small cell lung cancer patient receiving adjuvant chemotherapy is revealed for the first time, the detection kit is designed according to the discovery, and personalized guidance is provided for selecting the adjuvant chemotherapy scheme of the non-small cell lung cancer patient.