Digital Image Processing and Analysis with ImageJ

ImageJ is a freely available, cross-platform (e.g., Windows, Mac, Linux) image processing and analysis program developed by the NIH. In addition to being readily available for no cost, ImageJ is supported by a wide range of constantly evolving user-created functionalities to address a remarkable range of applications, complementing commercial software that typically comes with imaging instruments such as digital gel-imaging systems or microscopy workstations. New processing/analysis macros and plug-ins are routinely added to the support site, and are frequently validated via refereed publications. With the continued improvements and growth of fluorescence-based applications, ImageJ continues to be a mainstay in the laboratory. ImageJ has extensive support materials available online, its base code is regularly updated, and a survey of Medline references indicates that it is one of the most widely used image-analysis packages available today. Curr. Protoc. Essential Lab. Tech. 3:A.3C.1-A.3C.24. © 2010 by John Wiley & Sons, Inc. Keywords: computer graphics; image enhancement; image interpretation; imaging; microscopy; software; electrophoresis; gel analysis; fluorescence-based imaging; digital imaging; protein blotting