Effect of a monoclonal anti-mouse sperm antibody (OBF13) on the interaction of mouse sperm with zona-free mouse and hamster eggs

Abstract Mouse eggs freed from zonae by chymotrypsin were mixed with sperm and pro-nuclear formation was observed. When anti-mouse sperm monoclonal antibody (OBF 13) from ascites fluid was added to the medium (at a final concentration of 0.05%), fertilization was significantly inhibited (9.7 ± 4.3% compared to control 56.7 ± 7.4%, P