Regulation of BDNF Expression in Traumatic Brain Injury Rats Subjected Single Neucleated Cell Transplantation

Objective To investigate the effect of single neucleated cells transplantation on the neurological function and brain derived neurotrophic factor(BDNF) expression in traumatic brain injury(TBI) rats.Methods TBI rats were established by hammer fall method from 30 cm height with 50 g weight.Single neucleated cells/karyocytes from bone marrow were separated and prepared by ficoll medium,then transplanted into pericontusional tissues in the brain.Neurological function severity scores(NSS) were recorded at 1,3,and 7 days post operation(dpo).The pericontusional tissues were harvested at 7 dpo to analyze the BDNF localization,expressional level.Results Single neucleated cell transplantation decreased NSS significantly,compared with TBI rats without cell implantation(P0.05).BDNF expression was upregulated and mainly found in neurons.Conclusion The present study showed the single karyocytes transplantation could improve neurological function and the mechanism is possibly linked to the BDNF expression.