La modulación de la síntesis de IL-2 e IFN-γ por células mononucleadas humanas se asocia a la inmunosupresión fisiológica del fluido epididimario

Background and objective: IL-2 and IFN-γ play an important role in lymphocyte proliferation and activation. This study was undertaken to analyze if the inhibitory effect of the epididymal fluid on the immune response is associated with the modulation of the expression of IL-2 and IFN-γ. Material and methods: Mononuclear cells (Ficoll-paque d = 1.077) were isolated from blood from healthy donors and they were stimulated with epididymal fluid from everyone: an organ donor, and a man who was castrated by surgery. Once demonstrated the inhibition of proliferation of mononuclear cells, the levels of IL-2 and IFN-γ was determined by ELISA in supernatants of cultures of mononuclear cells stimulated with epididymal fluid and the results were expressed as inhibition or stimulation index. La modulacion de la sintesis de IL-2 e IFN-γ por celulas mononucleadas humanas se asocia a la inmunosupresion fisiologica del fluido epididimario Alain R Rodriguez-Orozco, MD, PhD;* Lorenzo E Mallea Sanchez, MD;** Ada J Machado Curbelo, MD;** Biologo, Victor Cabrera Oliva, PhD;*** Quimica, Maria de los A Carbonell Viamontes** Vol. 18, Num. 2 • Mayo-Agosto 2009 pp 39-46 www.medigraphic.org.mx