Prokaryotic expression and antiserum preparation of AC2 gene of Tomato yellow leaf curl Guangdong virus G3 isolate.

AC2 gene of Tomato yellow leaf curl Guangdong virus isolate G3(TYLCGuV-) was amplified from its recombinant plasmid by PCR,and the gene was cloned into prokaryote expression vector pET-28b(+).The expressed fusion protein was highly expressed in Escherichia coli Rosetta(DE3)Ⅲ induced by IPTG,and the molecular mass was about 19 500 by SDS-PAGE analysis.The fusion protein was used as an antigen to immunize the healthy rabbit and the antiserum of AC2 was prepared.The titer measured by ELISA was about 1∶ 16 384.Western-blotting analysis indicated that the antiserum could serologically react with AC2 protein of TYLCGuV-.