Study on the Regulating BDNF and mRNA of Neuron with TBI Rats Model in Different Periods

Objective To prepare TBI model of SD rat by the method,and study the effects of acupuncture treatment of TBI at different periods. To reveal protective effects and mechanism of the acupuncture to the injured nerve cells of TBI brain,provide experimental basis of acupuncture treatment of acute phase TBI at the best time,and to add the scientific contents of acupuncture treatment of TBI. Methods 70 SD rats were randomly divided into blank control group,acupuncture group 1,acupuncture group 2,acupuncture group 3 and model control group. The TBI animal models were prepared with the eCCI of the speed of 4m /s and the depth of 3mm. Than rats were treated by acupuncturing Baihui( DU20),Renzhong( DU26),the left Neiguan( PC6) and the left Zusanli( ST36)) after the brain injured at 1 day,3 days,5 days. The treatments of each group were repeated 10 times. The materials were collected at the 15 th day and to observe and examine the related index signs: By immunohistochemical and Western-blot to examine the localization and content of BDNF in the brain tissue protein,to detect the mRNA changes of BDNF by Fluorescence metered PCR method. Results The expression and content of BDNF were detected by immunohistochemistry and Western-blot in brain tissue. Acupuncture can up-regulate NGF. And by PCR,acupuncture can increase the mRNA of BDNF. After TBI occurred,while acupuncture treatment was used as soon as possible,the acupuncture was more obvious on the regulation effect. And there was significant differences among the three groups( P 0. 01). Conclusion Acupuncture treated TBI as early as possible.The results of this study showed that after 1 day of TBI occurred if acupuncture was used immediately,the curative effect was better than 3 days and 5 days latter.