DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION

The goal of the study is to establish a simple and precise HPLC method from its commercial formulation for the quantification of Apigenin and Luteolin. The separation of the analytes was performed by Hibar Lichrospher C8 (150 x 4.6mm i.d., 5μ) column with the mobile phase consisting of methanol and 0.5 % trifluroacetic acid (80:20 %, v/v) at 1 ml/min flow rate and 269 nm wavelength detection. The method developed was found to be linear over the concentration ranging from 10 to 110 ng/ml with > 0.99 correlation coefficient (R2) for both Apigenin and Luteolin. The results obtained from the validation studies states that the method developed was found to be simple, precise and accurate in accordance with the ICH guidelines. Through this original research work attempt was therefore made to standardize selected marketed formulations using apigenin and luteolin as an active biological marker for simultaneous quantification through established and validated HPLC techniques.