Establishment of Fingerprint of \Angzhi( Ganoderma lucidum Karst.)and Its Preparations by HPLC

Objective: To study the determination method of the fingerprint of lingzhi(Ganoderma lucidum) and its preparations and to establish the fingerprint of lingzhi and its preparations. Methods: The methanol extraction of lingzhi and its preparations was separated on a ZORBAX×300SB-C_(18) column(4. 6mm×250mm,5μm)elution using MeOH and 5%HAc-H_2O(40: 60) solvent system and detected at 252nm with a DAD detector. Flow rate was 0. 8mL·min~(-1) and 10μL was injected every time and the column temperature was 40℃. Results:The fingerprint of lingzhi and its preparations with 12 common peaks was established. The relative retention time was determined. Conclusion: The fingerprint can be used to distinguish lingzhi preparations. This method is quick, simple, repeatable, exact and need not any pretreatment. Therefore it provides scientific reference and effective determining ways for the quality control of lingzhi preparations.