Genetic Analysis of 23 Mango Cultivar Collection in Guangxi Province Revealed by ISSR

Twenty-three mango cultivars (Mangifera indica Linn) collecting in Guangxi province were examined by ISSR markers and two species relatives M. himalis J.Y. Liang and M. persiciformis Wu & Ming, other 15 mango cultivars from India (4), USA (3), Burma (3), Thailand (2), Philippines (1), Pakistan (1) and Sir Lanka (1) used as references. Of the 42 primers screened, 9 primers gave reproducible, polymorphic DNA amplification patterns, and were selected to construct a DNA fingerprinting map to distinguish the genotypes of mango. According to the banding patterns obtained with 9 selected primers, all cultivars tested in this study were distinguished from each other and showed ample genetic diversity, indicating that ISSR-PCR was an effective method for cultivar identification of mangoes. Based on 74 selected bands, all Guangxi mango cultivars tested were clustered into a big group with Neelum, Macheso, Aroemwnis, and Irwin by UPGMA analysis, indicating that Guangxi mango cultivars had a close relationship each other.