RP - HPLC METHOD DEVELOPMENT AND ITS VALIDATION FOR THE ESTIMATION OF FEXOFENADINE IN RAT PLASMA AND ITS APPLICATION FOR PHARMACOKINETIC STUD IES

A simple, sensitive and rapid reverse phase performance liquid chromatography (RP - HPLC) method was developed and validated for the determination of fexofenadine from small volumes of rat plasma. The Sample preparation was very simple and it involves protein precipitation method with ACN From the re sults of all the validation parameters and applicability of the assay . T he present method can be useful for pre - clinical pharmacokinetic studies of fexofenadine with desired precision and accuracy along with high - throughput. The developed analytical method is for fexofenadine in reverse phase chromatographic method utilizing C 18 column (250 mm × 4.6 mm , 5 µ). The peak was detected using a fluorescence detector set at Ex 220 nm and Em 280 nm . The mobile phase used was 1M triethylamine pH - 4.0: Acetonitrile in the ratio of 65:35. The retention time of fexofenadine was found to be 8.25 min utes . The total chromatogr aphic run time was 12.0 min utes . The flow rate of the mobile phase was 1.0 ml/min at room temperature . Method was found to be linear from 2 to 500 ng/ml for Fexofenadine (r 2 ≥ 0.9998) . The results indicate the bio - analytical method is linear, precise and a ccurate. The developed method was