SSR mapping of the Msf, a multiple-allele male-fertility restorer gene in Chinese cabbage (Brassica campestris L. ssp. pekinensis).

In order to construct a molecular-assisted selection(MAS)system for breeding the multiple-allele male-sterile lines in Chinese cabbage,a BC4 mapping populations was constructed with the fertile plant(MsfMsf) which was selfing progenies of the genetic multiple-allele male-sterile AB as the female parent and inbred line 'a20'(msms)which is the male parent.Screening 104 SSR primer pairs was performed with near isogenic lines(NILs).The results showed that three SSR markers named syau_m13,syau_m14 and BRMS-040 were found to be linked to the target Msf.Based on the genetic linkage analysis,the Msf gene was located on the linkege group R07.Finally,a genetic map of the Msf gene was constructed covering a total interval of 8.6 cM.The three markers were located on the same side of the gene at distances of 6.7,6.7 and 8.6 cM,respectively.