Polymorphism of intron 14 of porcine CACNA2D1 gene and effects on muscular pH45.

[Objective] To detect polymorphism of intron 14 of porcine CACNA2D1 gene and thus provide conditions for the marker-assisted selection of pork quality traits.[Method] The polymorphism of the exon 14 and intron 14 of porcine CACNA2D1 gene was detected in six breeds by PCR-SSCP.The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed.[Result] There were three kinds of genotypes,namely,AA,AG and GG,in the intron 14 of porcine CACNA2D1 gene.The polymorphic locus (G → A) was at the 1 145 nt of the sequence (GenBank Accepted No.FJ156361).The results of χ2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds (P0.05).The pH45 values of eye muscle and ham joint muscle were significantly different between genotype AA and genotype AG of pigs in F2 generation from the Jianhua×Pietrain resource family (P0.05).[Conclusion] The polymorphism of the intron 14 of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.