Establishment of a Sanguinarine-Producing Cell Suspension Culture of Argemone mexicana L (Papaveraceae): Induction of Alkaloid Accumulation

: A protocol for the induction of a cell suspension culture of Argemone mexicana is described. This suspension has been kept for over 3 years producing sanguinarine, a benzophenanthridine-type alkaloid. Sanguinarine levels can be increased by exposing these cultures to yeast or fungal elicitation.