Эффективность в отношении экспериментального геморрагического инсульта у крыс новой коллоидной формы низкосиалированного эритропоэтина на основе полилактидов

This study was aimed at developing a method for obtaining a nanosomal colloidal form of low-sialylated human erythropoietin (lsHEP) possessing neurotropic (antihemorrhage) activity. Parameters of HEP adsorption on nanoparticles of (i) homopolymer of lactic acid and (ii) copolymer of lactic and glycolic acids, average sizes of these nanoparticles, and aggregation stability of obtained complexes have been determined. The most effective adsorption (>80%) was achieved for a 50/50 copolymer of lactic and glycolic acids. Experiments on rats with intracerebral post-traumatic hematoma model showed that the obtained nanosomal lsHEP exhibited a significant neuroprotective effect, which was manifested by 40% reduced loss of test animals (77.8% survival). Chronic administration of nanosomal lsHEP did not influence hemopoiesis in the experimental animals.