Bioanalysis of small and large molecule drugs, metabolites, and biomarkers by LC-MS

Abstract Bioanalysis plays an important role in drug discovery and development. Bioanalytical scientists use sound scientific judgment, regulatory compliance, and project management to deliver pivotal data for project decisions in a timely manner. In this book chapter, we highlight the bioanalysis of small and large molecule drugs, metabolites, and biomarkers by LC-MS. We illustrate some of the challenges and problem-solving case examples. The case studies and literature are no doubt incomplete and biased toward our own experiences and publications. Nevertheless, we hope the readers can appreciate the complexity and dynamics of modern LC-MS-based bioanalysis.