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The Amazon is the largest biome in Brazil, covering the hydrographic basins of the Amazon River, Araguaia-Tocantins, coastal basins in the North West and coastal basins in the Northeast. The Puruzinho River micro basin is inserted in a region where there is an exclusive occurrence of the so-called Natural Fields. Thus, this work had a general objective to analyze the dynamic space-time of the vegetation indices in the detection of environmental transformations in a watershed of the Puruzinho River. The study area is located in Humaita, AM. The Puruzinho River basin is located in the Purus-Madeira interfluvium, Southwest of the Brazilian Amazon. The Model Maker tool of the ERDAS Imagine 2014 software was used to calculate the estimate of the Indices of vegetation and biophysicists. The highest average of the NDVI was for the image of the year 2010, presenting an average value of 0.354 and the lowest average of the NDVI was for the year 2017. The result of the standard deviation of the NDVI presented high values, varying between 0.278 for the image for the year 2017 and 0.358 for the year 2010. There is a predominance in the occurrence of the class represented by the light green colour for the years 2006, 2008, 2011 and 2017, revealing a predominance in the occurrence of less dense vegetation for those years. The biophysical indices showed an inverse pattern between the areas of dense forest and anthropized areas. Biophysical indexes, combined with remote sensing and geoprocessing techniques, proved to be an essential tool in monitoring and detecting areas with environmental changes.