Cloning,Sequence and Genetic Evolution Analysis of Mink β-interferon Gene

According to published GenBank sequences,a primer for the increase of mink β-interferon(IFN-beta) gene was designed and synthesized by DNAstart analysis software,total RNA was extracted from the infected mink spleen,mink β-interferon gene was increased about 561 bp through RT-PCR method.After this gene was cloned to pEasy-T1 carrier,the facts prove that this gene is mink β-interferon according sequence analysis.The result suggests that β-interferon nucleotide sequence homology are 100.0% and amino acid homology are also 100.0% when compared sequence result with published gene sequence(EF581890.1) from GenBank.The nucleotide sequence homology and amino acid homology are 83.0%~100.0% and 69.4%~100.0% respectively when compared with another canine animals.