89: Development of a Novel Micro LC-MS/MS Method for Assay of Agonist-Induced Release of Endogenous Serotonin from Human Platelets

Objective: Assessment of platelet function testing is often limited when platelet counts are low, as typically found in pediatric and thrombocytopenic populations. We aim to develop a high sensitive method for measuring the release of endogenous serotonin by mass spectrometry to address this limitation. Methods: 100 μL citrated blood was diluted 10-fold to 1.0 mL with Modified HEPES/Tyrode Buffer (MTB). 80 μL of the diluted blood was then incubated with a platelet agonist, such as ADP, unstirred, …