Hyperspectral Fourier transform spectrometer for spectral self-interference measurements of biological material on surfaces
2007
A hyperspectral Fourier transform spectrometer (HS-FTS) has been developed to study biological material binding to
surfaces through spatially resolved, spectral self-interference fluorescence microscopy and also label-free white light
reflectance spectroscopy. Spectral self-interference fluorescence microscopy yields the height of fluorescent tags bound
to a specific location on biomolecules tethered to a surface, and from this the biomolecule conformation can be
predicted; white light reflectance spectroscopy yields the average height of an ensemble of biomolecules relative to the
surface. The HS-FTS is composed of a small, step scanning Michelson interferometer made by Optra, Inc., a series of
commercial off the shelf imaging lenses, and a 12-bit thermoelectrically-cooled CCD camera. The system operates over
the 500 to 900 nm spectral range with user defined spectral resolution, thereby supporting use of a host of fluorescent
tags or white light spectral windows. The system also supports near real-time hyperspectral cube acquisition via
undersampling with the use of a spectral filter and user defined interferometer step increments. The overall approach
offers flexible yet sensitive measurement capability for a variety of biological studies. Preliminary results are presented
of both spectral self-interference fluorescence microscopy and white light reflectance spectroscopy measurements of
artificial, photographically etched surfaces with feature heights on the order of 10 nm. Planned future work includes
spectral self-interference fluorescence microscopy measurements of biomolecule conformation as manipulated by
external electrical and magnetic fields as well as label-free white light reflectance spectroscopy measurements of DNA
microarrays.
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