口蹄疫O/Taiwan/97病毒、豬水疱病病毒感染豬隻之臨床病理研究

Nineteen 30 to 50 kg body weight secondary specific pathogen free (SPF) pigs were divided into 2 groups and were artificially infected with the porcinophilic O/Taiwan/97 strain of foot- and-mouth virus (FMDV) and swine vesicular disease virus (SVDV), respectively. Serum samples of the pigs were collected on day 0, 1, 2, 3, 4, 5, 6, 8, 12, 15, 19, 26, 30, 44, and 50 days post- infection (DPI) for serum enzyme activity examination. Results showed that four enzyme activities released from damaged tissues, including creatine kinase (CK), lactate dehydronase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were detected in sera. In the group of pigs infected with FMDV, ALT activity increased on 1 DPI, and reached the peak on 5 DPI; AST activity increased sharply on 3 DPI and reached the peak around 4 to 5 DPI; CK activity increased sharply on 4 DPI and reached the peak on 5 DPI; LDH activity increased on 5 DPI and remained at high level from 6 to 15 DPI. On the other hand, in the group of pigs infected with SVDV, activities of AST, ALT, and LDH were found slightly increasing on 2, 3, 5, and 8 DPI; the CK activity did not show any significant changes. It can be seen that the activities of these four enzymes will be released substantially from the damaged muscles into serum after infection with the OlTaiwan/97 strain of FMDV. Thus, we suggest that early and differential diagnosis of FMD and SVD can be accomplished by measuring the activities of CK, LDH, AST, and ALT.