Recombinant mutant human TRAIL inhibits growth of non-small cell lung cancer cell lines in vitro

Objectives To investigate the inhibitory effects of recombinant mutant human TRAIL (rmhTRAIL) on human NSCLC cells and normal cells and the combined efficacy with chemo-therapy medicine. Methods The growth of cells was examined with MTT assay and colony forming assays. We also observed the synergy of rmhTRAIL and cisplatin (DDP) on growth of cells. Results rmhTRAIL has significant inhibitory effects on several NSCLC cells, but not on human normal cells. Synergic or enhanced inhibitory effects on NSCLC cells were also observed when rmhTRAIL was used together with DDP and there was no toxicity on human normal cells. Conclusion rmhTRAIL is a safe and effective anti-tumor bio-product.