Chiral Separation of Cationic Drugs on an α1-Acid Glycoprotein Bonded Stationary Phase

Abstract A commercially available chiral stationary phase containing α1-acid glycoprotein on silica (EnantioPac, LKB) was applied to the resolution of a number of pharmacologically important enantiomeric ammonium compounds. The optimization of retention and selectivity by cationic, anionic and neutral modifiers in the mobile phase was studied. The results suggest that the solutes are retained according to an ion-pair distribution model. Compounds of widely different structures were studied, and high separation factors were achieved for a majority.