Identification of O-GlcNAc modified osteoblast proteins by electron transfer dissociation tandem mass spectrometry reveals proteins critical for bone formation

Abbreviations: LWAC, lectin weak affinity chromatography; ETD, electron transfer dissociation; CID, collision induced dissociation; CBP, CREB-binding protein; RUNX2, runt-related transcription factor 2; OGT, O-GlcNAc transferase; OGA, O-GlcNAcase; O-GlcNAc, O-linked Nacetylglucosamine; WGA, wheat germ agglutinin; ALP, alkaline phosphatase; OCN, osteocalcin; PTH, parathyroid hormone; IGF-1, insulin-like growth factor 1; BMP, bone morphogenetic protein.