근경 배양을 이용한 알스트로메리아 우량 육종계통 C269의 대량증식 체계 개발

An in vitro production system of Alstroemeria C269 was investigated to provide virus-free stock of domestically selected for cultivar registration. Alstroemeria C269 was bred in the ornamental plant science laboratory at Chonnam National University and primarily cultured on MS basal medium. Rhizome tips for rhizome mass propagation were cultured on MS medium supplemented with different concentrations of 6-benzylaminoputin (BAP) and kinetin (KIN ). E xplants from t he m edium supplemented w ith KIN had numerous shoots and densely grew. Therefore, this approachwas unsuitable for in vitro rhizome mass propagation due to difficult rhizome splitting. However, explants cultured in medium supplemented with BAP at a concentration below 0.2 mg L-1 were appropriate for in vitro rhizome split propagation. The greatest number of roots was obtained from the medium supplemented with NAA at 0.25 mg L-1. However, abnormal roots were observed in the medium supplemented with NAA. More rootlets and root hair were induced in MS medium than in MS medium supplemented with NAA. In acclimation, plantlets survived more in MS medium t han in M S medium s upplemented with N AA. Besides, the shoot induction and aboveground growth were better in MS medium than in MS medium supplemented with NAA. Based on these results, the efficient rhizome mass propagation method of Alstroemeria hybrid C269 is 8 weeks of culture in MS medium supplemented with 0.2 mg L-1 BAP, followed by culture in MS medium for around 5 weeks for rooting before acclimation.