The sustaining effects of e-waste-related metal exposure on hypothalamus-pituitary-adrenal axis reactivity and oxidative stress.

Abstract Taizhou is one of the three largest electronic waste (e-waste) recycling locations in China. At present, to prevent the environmental problems stem from e-waste dismantling, the local government shut down all the industries in 2015. However, little is known to the sustaining effects of e-waste exposure on human health after the e-waste recycling factories were closed. In this study, we collected blood samples in the residents living near e-waste dismantling factories (exposed group) and a chosen reference area (reference group) for comparison in Taizhou in December 2017.17 metals were quantitatively determined in all blood samples. Among them, the concentrations of altogether 9 metals, including chromium (Cr), arsenic (As), cobalt (Co), nickel (Ni), silver (Ag), stannum (Sn), mercury (Hg), lanthanum (La) and cerium (Ce) were significant higher in the individuals in exposed group than in reference group. Hypothalamus-pituitary-adrenal axis (HPA axis) is critical endocrine system in regulating many physical functions. We found that the concentrations of hormones in HPA axis, including corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and cortisol, were higher in exposed group than in reference group. Moreover, we also observed significantly higher concentrations of biomarkers of oxidative stress (OS), including malondialdehyde (MDA) and 8-isoprostane (8-I), in exposed group compared with reference group even though the e-waste industries have been shut down for over 2 years. Meanwhile, the hormones in the HPA axis and the biomarkers of OS showed sinificantly positive correlation. The results of Pearson correlation and multiple linear regression showed that blood Cr and Ni positively correlated with the 3 hormones and 2 biomarkers of OS. Considering the relationship between Ni and Cr, HPA axis, OS, we speculated that high concentrations of Cr and Ni exposure could induce oxidative damage in e-waste exposure persons, and the regulations of HPA axis could play the important role during the process.