Preparation and comparison of three different adjuvant inactivated vaccines of Japanese encephalitis virus from swine.

The oil-emulsion,propolis and 603 nanometer inactivated vaccines of Japanese encephalitis virus GZ0409-31 strain from swine were prepared and compared by sterility and safety test.Mice immunized with these inactivated vaccines displayed effective immune protection that testifies their safety.The groups of mice immunized with the oil-emulsion and 603 nanometer inactivated vaccines had higher antibody titer until 90 days post inoculation whereas the group of mice immunized with attenuated Japanese encephalitis vaccine had lower antibody titer.In addition the level of IFN-γ was higher in the group of mice immunized with attenuated Japanese encephalitis vaccine than those groups with inactivated Japanese encephalitis vaccines.The group of mice immunized with 603 nanometer inactivated Japanese encephalitis vaccines expressed higher level of IFN-γ than those other inactivated Japanese encephalitis vaccines.Mouse immunized with the oil-emulsion,propolis and 603 nanometer inactivated vaccines could be protected from the infection of GZ0409-31 Japanese encephalitis virus with 100% protection rate in case of oil-emulsion and 603 nanometer inactivated vaccines immunized groups but remained 70% in propolis inactivated vaccines immunized groups.The attenuated Japanese encephalitis vaccine also provided 100%(10/10)protection rate.The oil propolis and 603 nanometer inactivated GZ0409-31 Japanese encephalitis vaccines were prepared and found to be safe and effective.