Optimization of supercritical CO2 fluid extraction process for polysaccharide from aloe.
2011
[Objective] To optimize the supercritical CO2 fluid extraction process for aloe polysaccharide, so as to obtain high-quality aloe polysaccharide. [Method] The static and dynamic extraction duration were optimized by single factor test, and the extraction kettle conditions were optimized by orthogonal test. [Result] The optimum supercritical CO2 fluid extraction conditions were as following: 2.5 ml/g of ethanol amount, extraction pressure of 25 MPa and extraction temperature of 35 ℃. In addition, the optimal static and dynamic extraction duration were 60 and 30 min, individually. Under the optimal conditions, the yield of aloe polysaccharide reached as high as 85.10%. [Conclusion] Gentle in extraction conditions, the environment-friendly and energy-saving supercritical CO2 fluid extraction technology is favorable for extracting polysaccharide from aloe.
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