Engagement of CD81 induces ezrin tyrosine phosphorylation and its cellular redistribution with filamentous actin

Engagement of CD81 induces ezrin tyrosine phosphorylation and its cellular redistribution with filamentous actin Greg P. Coffey * , Ranjani Rajapaksa * , Raymond Liu * , Orr Sharpe † , Chiung-Chi Kuo * , Sharon Wald Krauss ‡ , Yael Sagi * , R. Eric Davis § , Louis M. Staudt § , Jeff P. Sharman * , William H. Robinson † , and Shoshana Levy * Stanford University, School of Medicine, Division of Oncology, Stanford CA 94305, USA Stanford University, School of Medicine, Division of Immunology and Rheumatology, GRECC Veterans Affairs Palo Alto Health Care System, Palo Alto CA, 94304, USA Life Sciences Division, University of California Lawrence Berkeley National Laboratory, Bldg 74, 1 Cyclotron Rd, Berkeley, CA 94720, USA. Metabolism Branch, National Cancer Institute, Bethesda, MD 20892, USA Short title: CD81 induction of ezrin phosphotyrosine Keywords: tetraspanins, signal transduction, Syk. Corresponding Author: Shoshana Levy, Ph.D. Division of Oncology, Department of Medicine CCSR Room 1105a, 269 Campus Drive, Stanford CA, 94305 Phone: 650-725-6425 Fax: 650-736-1454 Email: slevy@stanford.edu Abbreviations: B cell receptor (BCR); ezrin-radixin-moesin (ERM); filamentous actin (F-actin); fluorescein isothiocyanate (FITC); isotype control (IC); liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) monoclonal antibodies (mAbs); peripheral blood mononuclear cells (PBMC); spleen tyrosine kinase (Syk); tetramethyl rhodamine iso-thiocyanate (TRITC).