An ultrasensitive and visible lighting-up probe for imaging thiophenols in water samples, in serum and visualizing thiophenols-induced oxidative stress process in live cells

Abstract Thiophenols, a class of significant industrial materials, are extremely toxic in environmental as well live cells. However, the process of live cells responding to thiophenols is not well understood. Herein, an innovative “OFF-ON” probe FY for thiophenols selectively in 100% aqueous solution was reported. It featured rapid response (~150 s), prominent sensitivity (detection limit: 5 nM), and large Stokes shift (~104 nm), which assured specific detection of thiophenols in A375 cells, HeLa cells and environment. Especially, it proved that thiophenols in live cells can be eliminated by endogenous reactive oxygen species (ROS), indicating that thiophenols may result in cellular oxidative stress. As well, it was resoundingly put into recognizing of thiophenols quantitatively in actual water samples and in serum.