Effect of Acid Mine Drainage of Coal Mining on Total Protein Content of Muscle and Liver in a Freshwater Fish, Channa punctatus (Bloch)

The paper deals with the studies on impact of Acid Mine Drainage of coal mining on total soluble protein content of fish, Channa punctatus. Acid Mine Drainage of coal mines was collected from the coal mining area near Simsang River, Garohills, Meghalaya and LC50 value (28 % of effluent) for 96 h was determined. Fishes were treated in sub lethal concentration (one-fifth of LC50 value) of Acid Mine Drainage of coal mines for 24, 48, 72 and 96 h to evaluate its impact on total soluble protein in the muscle and liver. It was found that there was a decreasing trend of total protein content and maximum decrease was found at 48 and 72 h in liver and muscle respectively. However, a little rise of protein content was found at 72 h in liver and 96 h in muscle. This might be due to rise of proteolytic activity in liver suggesting the increased enzymatic activities to cope with toxic stress.