Effects of Lonicerae Caulis (LC) on Gene Expression of Human melanoma cells

Dae-Su Kim․ Jin-Soo Song․Min-Yeong Jung․Min- Jeong Kim․Soo-Yeon Park․Jeong-Hwa Choi․Jong-Han Kim Objective : This study was designed to investigate anti-cancer and whiteni ng activities of Lonicerae Caulis (LC). So it was investigated the effects of LC on proliferation rates of melanoma cells, then effects on genetic profile by LC. Methods : The genetic profile for the effect of LC on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysi s on these genes were conducted. Total 441 genes were up-regulated and 830 genes down-regulated in cells t reated with LC. Genes induced or suppressed by LC were all mainly concerned with basic signallin g pathways, which are involved in cell growth, differentiation and migration. Especially, many genes, which are related in apoptosis and cell cycle arrest were up-regulated by treatment with LC, and genes related in cell cycle were down-regulated. Result : The network of total protein interactions were identified by using cytoscape program, and some key molecules, such as BCL2L1, SIN3A, SMAD2 and c-myc that can be used for elucidation of therapeutical mechanism of medicine in the future. Conclusion : These results suggest possibility of LC as anti-cancer drug and whitening cosmetics. In addition, it was also suggested that related mechanisms are inv olved in BCL2L1, SIN3A, SMAD2 and c-myc related signalling pathways.Key words : 忍冬藤, 악성 흑색종, DNA chip