GISH analysis of Thinopyrum intermedium

Thinopyrum intermedium (Th.intermedium) was analysed by Genomic in situ hybridization (GISH) with the probes of total genomic DNA of Th.elongatum 2n=2x=14,E eE e, Th.bessarabicum 2n=2x=14,E bE b,and Pseudoroegneria strigosa (Ps.strigosa ),2n=4x=28,S tS tS tS t respectively.The results showed that the genome of Th.intermedium consists of two close related genomes similar to the genome of Th.elongatum and Th.bessarabicum and one distant genome similar to Ps.strigosa .Thus Th.intermedium has the genome formula E e tE e tE b tE b tS tS t.