Localization of acetylated histone H4 in the macronucleus of Euplotes
1991
Antisera specific to acetylated and unacetylated N-terminal domains of histone H4 were employed to map the spatial distribution of exposed antigenic determinants in the macronucleus of Euplotes eurystomus. Sites of putative transcription-related acetylation appear to reside in the regions between condensed chromatin granules and disappear during chromatin restructuring in the forward zone of replication bands. Coincident with synthesis of newly replicated chromatin, the specific antisera reveal a resumption of exposed sites of H4 acetylation in the rear zone of replication bands.
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