SPME-GC-MS determination of phenol in the smoke of moxa

Objective:To establish a solid-phase microextraction(SPME)-GC-MS method for the determination of phenol in combustion product of moxa-moxa smoke.Methods:A DB-5MS(30 m×0.25 mm×0.25 μm) capillary column was adopted with programming temperature.Helium was used as carrier gas.Ionization mode was EI source.Selected ion monitoring mode was used with the target ion of m/z 94.Results:The calibration curve was linear in the range of 0.0063-0.0295 mg·L-1(r=0.9964);The average recovery was 94.0%(RSD=3.0%,n=5).The generated content of the phenol was between 0.679-2.238 mg·g-1.The content of phenol in the moxa smoke was between 8.3-27.2 mg·mL-1.Conclusion:This method is sensitive,accurate,repeatable,and easy to operate,which can be used for the phenol content of combustion product of moxa smoke.The content of phenol in moxa smoke are conform the national standard,it provided a basis for the quantitative analysis and security research of moxa smoke.