Anti-proliferative activity of Gum kondagogu (Cochlospermum gossypium)-gold nanoparticle constructs on B16F10 melanoma cells: An in vitro model

Abstract The present study has explored the reducing and capping potentials of gum kondagogu (GK) for the synthesis of gold nanoparticle constructs. The formation of gold nanoparticles was confirmed by UV- absorption, FTIR, AFM, SEM and TEM. The GK-AuNPs showed a characteristic absorption peak at 534 nm in UV spectra with the size range of 4.08–12.73 ± 0.75 nm. As a result of wide screening on the application of newly synthesized GK-AuNPs their anti-proliferative potential were discovered by MTT assay, confocal microscopy, flow cytometry and quantitative real time PCR techniques. MTT assay of synthesized GK-AuNPs exhibited IC 50 concentration of 1.7 µg L −1 after 48 h. FACS analysis revealed that cell cycle gets arrested at G1 to S phase, with up-regulation of p53, caspase-3, caspase-9, PPARa and PPARb genes and down regulation of Bcl-2 and Bcl-x(K) genes, in B16F10 cells treated with GK-AuNPs confirming the anti-proliferative properties of the nanoparticles.